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SPERM PROCESSING KITS FOR IUI


Sperm Processing is the cornerstone for intra-uterine insemination and other assisted reproductive techniques CRYOBANK offers ready-to-use kits which enable gynecologists to process semen even in modest clinical set-ups without elaborate laboratory facilities. The kits are safe, efficient, economical and convenient to use. Each kit is designed for processing of one semen sample; therefore, there is no chance of cross contamination or change of pH. The shelf life of the kit is 12 months when stored at 2 to 8 degrees Celsius.

All media tests done are: pH, Osmolarity, sterility, endotoxin levels, sperm survival tests ... etc.

Advantages of CRYOBANK Media products:

  Ready to use for laboratory convenience.
  Packaged in single-use ampoules, avoiding contamination of left-over media.
  Formulated with components designed to improve product stability and longer shelf-life.

Density Gradient-70%(2ml)

This consists of a set of two ampoules. One contains density gradient-70% solution for density separation and the other HTF solution for washing and incubation of sperm. Density gradient is a solution of silane- coated silica particles in HEPES-buffer and other capacitating factors.
Density Gradient-70%(2ml)
EFFECTIVE FOR :

This kit is effective for all types of sperm count -high count or low count, abnormal sperm, debris, round cells and viscous.
Components:

Silane-Coated Silica, KCl
NaCl
Glucose, Lactate
Na Pyruvate, HEPES
EDTA, H2O

HTF Solution (5ml)

HTF solution is the Human Tubal Fluid media with HEPES buffer, Human Serum Albumin (0.5%) and antibiotics. This modified HTF provides optimum pH maintenance over the physiological range (7.2 to 7.4) without the need for CO2 incubator. Therefore, it is widely used for washing and short-term incubation where a CO2 incubator is not available.
HTF Solution (5ml)
Components:

NaCl, Pyruvate, KCl, EDTA, KH2PO4, H2O
Glucose, HEPES sodium salt, NaHCO3,
MgSO4, has(human serum albumin-5%),Alanye Gultamine,

Density Gradient 90%-(1ml)

This consists of a set of three ampoules. One contains density gradient-90% and 45%, the other HTF solution for washing and incubation of sperm
Density Gradient 90%-(1ml)
Density Gradient 45%-(1ml)
EFFECTIVE FOR :

This kit is specially effective for contaminated semen sample and highly viscous sample, pus cells, round cells, dead cells, abnormal sperm, debris, high counts. Suggest for better results.

Components:

Silane-Coated Silica, KCl
NaCl
Glucose, Lactate
Na Pyruvate, HEPES
EDTA, H2O

Component Media

Cryobank also offers several ready-to-use component media in small convenient packing of 5ml in fine grade hermetically sealed ampoules.

HAM F- 10 (5ml) :

HAM F-10 is a modification of basic HAM F-10 with HEPES (21mM), Pyruvate, Human Serum Albumin (0.5%) . The addition of HEPES buffer maintains the pH over physiological range (7.2 to 7.4).

EFFECTIVE FOR :

HAM F-10 media is effective for normospermic semen sample-for semen separation and washing spermatpzoa. Specially and stability. Also swimup technique.

EBSS With HSA (5ml) :

On request EBSS(Earles Balanced Salt Solution with HEPES Buffer, Human Serum Albumin) are available.

EFFECTIVE FOR :

It is also swimup technique.